Zhang Xiaolan, Guo Peng, Jing Guozhong
National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing 100101, P.R. China.
Biotechnol Lett. 2003 May;25(10):755-60. doi: 10.1023/a:1023563600459.
An expression vector, pET-DB, with a perfectly matching downstream box of the initiation codon has been constructed on the basis of the pET system. Any gene of interest can then be inserted into the vector. Four genes were used to test the expression efficiency of the vector. The results show that the vector pET-DB can further increase protein expression level at least up to 35-70% as compared with the initial T7 expression system, indicating that the downstream box can enhance protein expression in Escherichia coli.
基于pET系统构建了一种表达载体pET-DB，其起始密码子的下游框完全匹配。然后可将任何感兴趣的基因插入该载体。使用了四个基因来测试该载体的表达效率。结果表明，与最初的T7表达系统相比，载体pET-DB可将蛋白质表达水平进一步提高至少35%至70%，这表明下游框可增强大肠杆菌中的蛋白质表达。
