使用ω-转氨酶/醇脱氢酶/葡萄糖脱氢酶偶联反应从外消旋α-甲基苄胺同时合成对映体纯的(R)-1-苯乙醇和(R)-α-甲基苄胺。
Simultaneous synthesis of enantiomerically pure (R)-1-phenylethanol and (R)-alpha-methylbenzylamine from racemic alpha-methylbenzylamine using omega-transaminase/alcohol dehydrogenase/glucose dehydrogenase coupling reaction.
作者信息
Yun Hyungdon, Yang Yung-Hun, Cho Byung-Kwan, Hwang Bum-Yeol, Kim Byung-Gee
机构信息
School of Chemical Engineering and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea.
出版信息
Biotechnol Lett. 2003 May;25(10):809-14. doi: 10.1023/a:1023500406897.
A simultaneous synthesis of (R)-1-phenylethanol and (R)-alpha-methylbenzylamine from racemic alpha-methylbenzylamine was achieved using an omega-transaminase, alcohol dehydrogenase, and glucose dehydrogenase in a coupled reaction. Racemic alpha-methylbenzylamine (100 mM) was converted to 49 mM (R)-1-phenylethanol (> 99% ee) and 48 mM (R)-alpha-methylbenzylamine (> 98% ee) in 18 h at 37 degrees C. This method was also used to overcome product inhibition of omega-TA by the ketone product in the kinetic resolution of racemic amines at high concentration.
利用ω-转氨酶、醇脱氢酶和葡萄糖脱氢酶，通过偶联反应实现了从外消旋α-甲基苄胺同时合成(R)-1-苯乙醇和(R)-α-甲基苄胺。在37℃下反应18小时，100 mM的外消旋α-甲基苄胺转化为49 mM的(R)-1-苯乙醇（对映体过量>99%）和48 mM的(R)-α-甲基苄胺（对映体过量>98%）。该方法还用于在高浓度外消旋胺的动力学拆分中克服酮产物对ω-转氨酶的产物抑制。
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