通过共表达ω-转氨酶和乙酰乳酸合酶的重组大肠杆菌不对称合成(S)-α-甲基苄胺。

Asymmetric synthesis of (S)-alpha-methylbenzylamine by recombinant Escherichia coli co-expressing omega-transaminase and acetolactate synthase.

作者信息

Yun Hyungdon, Kim Byung-Gee

机构信息

School of Biotechnology, Yeungnam University, Gyeongbuk, Korea.

出版信息

Biosci Biotechnol Biochem. 2008 Nov;72(11):3030-3. doi: 10.1271/bbb.80410. Epub 2008 Nov 7.

DOI:10.1271/bbb.80410

PMID:18997410

Abstract

To produce (S)-alpha-methylbenzylamine (MBA) from acetophenone, recombinant Escherichia coli co-expressing omega-transaminase and acetolactate synthase was used as a whole-cell biocatalyst. The solvent-bridge reaction system increased the yield of the whole-cell reaction by 2.5-fold, and the inhibitory (S)-alpha-MBA produced in the omega-transaminase reaction solution (pH 8.0) moved into the extraction solution (pH 3.0) via an organic solvent.

摘要

为了从苯乙酮生产(S)-α-甲基苄胺(MBA)，共表达ω-转氨酶和乙酰乳酸合酶的重组大肠杆菌被用作全细胞生物催化剂。溶剂桥反应体系使全细胞反应的产率提高了2.5倍，并且在ω-转氨酶反应溶液(pH 8.0)中产生的抑制性(S)-α-MBA通过有机溶剂转移到萃取溶液(pH 3.0)中。