Lyamina Nadezhda P, Dolotovskaya Polina V, Lyamina Svetlana V, Malyshev Igor Y, Manukhina Eugenia B
Saratov Research Institute of Cardiology, Saratov, Russia.
Med Sci Monit. 2003 Jul;9(7):CR304-10.
Young individuals with high normal blood pressure (HNBP) are at risk for hypertension. The aim of our study was to compare NO production and the intensity of free radical processes in young males with different BP levels.
MATERIAL/METHODS: Male subjects aged 18-45 years with normal BP, HNBP, and hypertension underwent physical and cardiological examination. NO production was evaluated by measuring plasma nitrite and nitrate (NOx) and 24-hour urinary excretion of NOx. Lipid peroxidation (LP) intensity and serum antioxidant activity (AOA) were measured using the biochemiluminescence method.
HNBP was associated with increased 24-hour systolic BP (SBP), diastolic BP (DBP), SBP and DBP variability, plasma and 24-hour urinary NOx and LP intensity, and decreased total AOA as compared to normotensive controls. We observed a direct nonlinear correlation between plasma NOx and SBP and between 24-hour urinary NOx and DBP, and a close inverse correlation between LP intensity and AOA in patients with HNBP. In the presence of two cardiovascular risk factors (smoking and obesity), patients with HNBP displayed higher LP intensity and lower levels of NOx than in both nonsmokers with normal body weight and control subjects. In hypertensive patients, SBP, DBP and LP intensity inversely correlated with plasma and urinary NOx.
Activation of LP processes and depression of AOA proceed in parallel with declining NO production and severity of hypertension. Early correction of the revealed disorders before the appearance of clinical symptoms may be promising in terms of prevention and treatment of cardiovascular disease.
血压处于正常高值（HNBP）的年轻人有患高血压的风险。我们研究的目的是比较不同血压水平的年轻男性体内一氧化氮（NO）的生成情况以及自由基过程的强度。
材料/方法：对年龄在18 - 45岁、血压正常、处于正常高值血压和患有高血压的男性受试者进行体格检查和心脏检查。通过测量血浆亚硝酸盐和硝酸盐（NOx）以及24小时尿中NOx排泄量来评估NO生成情况。采用生物发光法测量脂质过氧化（LP）强度和血清抗氧化活性（AOA）。
与血压正常的对照组相比，正常高值血压与24小时收缩压（SBP）、舒张压（DBP）升高，SBP和DBP变异性增加，血浆和24小时尿中NOx以及LP强度增加，总AOA降低有关。我们观察到在正常高值血压患者中，血浆NOx与SBP之间、24小时尿中NOx与DBP之间存在直接的非线性相关性，LP强度与AOA之间存在密切的负相关性。在存在两种心血管危险因素（吸烟和肥胖）的情况下，正常高值血压患者的LP强度高于体重正常的非吸烟者和对照组，且NOx水平较低。在高血压患者中，SBP、DBP和LP强度与血浆和尿中NOx呈负相关。
LP过程的激活和AOA的降低与NO生成的减少以及高血压的严重程度同步发生。在临床症状出现之前对所发现的紊乱进行早期纠正，对于心血管疾病的预防和治疗可能具有前景。
