Rassokhin A G, Pochinskiĭ A G, Liannoĭ K V, Ovchinnikov G A
Biull Eksp Biol Med. 1992 Oct;114(10):347-51.
A microfluorimetric system was used to study rat bone marrow erythroblastic islands (EI) and macrophages. Measurement of fluorescence from acridine-orange-treated cells was performed on the microscope LUMAM-E3 (LOMO, Leningrad) at 530-550 and 630-650 nm. The intensity of fluorescence in 530-550 nm range depended on the intensity of proliferative processes in EI, that at 630-650 nm was associated with activity of lysosomal apparatus of EI macrophages. Erythroblast amplification in EI is parallel both to enhancement of fluorescence intensity at 530-550 nm and 630-650 nm. Intensity of fluorescence of different bone marrow macrophages was evaluated. It is suggested that a part of bone marrow macrophages has high affinity to erythroid tissue.
使用微荧光测定系统研究大鼠骨髓红细胞生成岛(EI)和巨噬细胞。在显微镜LUMAM-E3(列宁格勒的LOMO公司)上,于530 - 550纳米和630 - 650纳米处对吖啶橙处理过的细胞进行荧光测量。530 - 550纳米范围内的荧光强度取决于EI中增殖过程的强度,而630 - 650纳米处的荧光强度与EI巨噬细胞溶酶体装置的活性相关。EI中幼红细胞的扩增与530 - 550纳米和630 - 650纳米处荧光强度的增强是平行的。评估了不同骨髓巨噬细胞的荧光强度。有人提出,一部分骨髓巨噬细胞对红系组织具有高亲和力。