一种用于检测牛κ-酪蛋白基因等位基因变体的聚合酶链反应-限制性片段长度多态性检测法。

A PCR-RFLP test to detect allelic variants of the bovine kappa-casein gene.

作者信息

Soria L A, Iglesias G M, Huguet M J, Mirande S L

机构信息

Laboratory of Genetics, Department of Animal Production, School of Veterinary Science, University of Buenos Aires, Buenos Aires, Argentina.

出版信息

Anim Biotechnol. 2003 May;14(1):1-5. doi: 10.1081/ABIO-120020180.

DOI:10.1081/ABIO-120020180

PMID:12887174

Abstract

Point mutations in exon IV of bovine kappa-casein gene (kappaCn, CASK, CSN3) determine nine allelic variants (A, B, C, E, F, G, H, I, and A1) for the gene. These variants are associated with major differences in composition and manufacturing properties of milk (i.e., cheese yield). A PCR-RFLP test was developed in order to distinguish the different alleles. Polymorphisms are detected by digestion with the endonucleases HindIII, HaeIII, and MaeII followed by electrophoresis in agarose gels stained with ethidium bromide. Twenty eight DNA samples from different breeds of Argentina were analyzed for the A, B, and E variants. This simple PCR-RFLP test makes feasible the inclusion of kappa-casein genotypes in breeding plans.

摘要

牛κ-酪蛋白基因（κCn、CASK、CSN3）外显子IV中的点突变决定了该基因的9个等位基因变体（A、B、C、E、F、G、H、I和A1）。这些变体与牛奶的成分和加工特性（即奶酪产量）的主要差异有关。为了区分不同的等位基因，开发了一种PCR-RFLP检测方法。通过用核酸内切酶HindIII、HaeIII和MaeII消化，然后在溴化乙锭染色的琼脂糖凝胶中进行电泳来检测多态性。对来自阿根廷不同品种的28个DNA样本进行了A、B和E变体分析。这种简单的PCR-RFLP检测方法使得在育种计划中纳入κ-酪蛋白基因型成为可能。