Mitra A, Schlee P, Krause I, Blusch J, Werner T, Balakrishnan C R, Pirchner F
Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal, India.
Anim Biotechnol. 1998;9(2):81-7. doi: 10.1080/10495399809525896.
We screened 57 Sahiwal cattle (Bos indicus) and 53 Murrah, 19 Nili-Ravi and 11 Egyptian buffaloes (Bubalus bubalis) to detect the polymorphisms at kappa-casein (CSN3) gene using the polymerase chain reaction (PCR). CSN3 A and B alleles were identified by PCR-RFLP using the restriction enzymes that detect the underlying nucleotide changes at codon 136 (Taql) and at codon 148 (HindIII or HinfI) in cattle. The frequency of CSN3 B allele in the Sahiwal cattle was estimated as 0.16 with no homozygous BB animal. Using the same set of primers as used in the Sahiwal cattle, a part of exon IV of buffalo CSN3 gene was amplified, but restriction enzyme analysis using HindIII/HinfI and TaqI did not reveal any polymorphism. However, DNA sequencing of the amplified fragment (GenBank Acc. No. U96662) revealed one polymorphism at codon 135 (ThrACC -->I1eATC) in buffalo; the frequencies of 135 Thr/Ile alleles were estimated as 0.88 and 0.12, respectively.
我们使用聚合酶链反应（PCR）对57头萨希瓦尔牛（印度瘤牛）、53头摩拉水牛、19头尼里-拉菲水牛和11头埃及水牛（水牛属）进行筛选，以检测κ-酪蛋白（CSN3）基因的多态性。通过PCR-RFLP，使用能检测牛中第136位密码子（Taql）和第148位密码子（HindIII或HinfI）潜在核苷酸变化的限制性内切酶来鉴定CSN3 A和B等位基因。萨希瓦尔牛中CSN3 B等位基因的频率估计为0.16，没有纯合BB动物。使用与萨希瓦尔牛相同的引物对，扩增了水牛CSN3基因外显子IV的一部分，但使用HindIII/HinfI和TaqI进行的限制性酶切分析未发现任何多态性。然而，扩增片段的DNA测序（GenBank登录号U96662）显示水牛在第135位密码子（ThrACC -->IleATC）处存在一个多态性；135 Thr/Ile等位基因的频率分别估计为0.88和0.12。
