对新型单克隆抗体2C9所识别表位的进一步研究。
Further investigation of the epitope recognized by the new monoclonal antibody 2C9.
作者信息
Kato Keitaro, Yoshikawa Kazuhiro, Taki Tomohiro, Shitara Kenya, Nakamura Kazuyasu, Hirota Maiko, Hanai Nobuo, Nakamura Kogenta, Kokubo Hiroto, Mitsui Kenji, Yamada Yoshiaki, Honda Nobuaki, Ueda Ryuzo, Saga Shinsuke, Fukatsu Hidetoshi
机构信息
Department of Urology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan.
出版信息
Int J Urol. 2003 Aug;10(8):439-44. doi: 10.1046/j.1442-2042.2003.00655.x.
OBJECTIVE
We established a new monoclonal antibody (2C9) that reacted with prostate tissue. The immunohistochemical reactivity of this antibody is similar to anti-prostate-specific membrane antigen (PSMA). Herein, we report the antigenic determinant of 2C9 antibody.
METHODS
The reactivity of the antibody was characterized by immunohistochemical staining and the antigen target was characterized by amino acid sequencing after immuno-affinity purification from an LNCaP cell lysate and cloning of a cDNA using a mammalian expression cDNA cloning system.
RESULTS
The amino acid and nucleotide sequences for the antigen molecule recognized with 2C9 monoclonal antibody demonstrated identity with PSMA.
CONCLUSION
The target molecule of the 2C9 monoclonal antibody is PSMA, pointing to future diagnostic and therapeutic applications.
目的
我们制备了一种与前列腺组织反应的新型单克隆抗体（2C9）。该抗体的免疫组化反应性与抗前列腺特异性膜抗原（PSMA）相似。在此，我们报告2C9抗体的抗原决定簇。
方法
通过免疫组化染色表征抗体的反应性，并通过从LNCaP细胞裂解物中进行免疫亲和纯化以及使用哺乳动物表达cDNA克隆系统克隆cDNA后进行氨基酸测序来表征抗原靶点。
结果
用2C9单克隆抗体识别的抗原分子的氨基酸和核苷酸序列与PSMA相同。
结论
2C9单克隆抗体的靶分子是PSMA，这为未来的诊断和治疗应用指明了方向。
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