Donn F, Bruns T, von Meyerinck L, Schulz M, Becker W M, Becker H, Klosterhalfen H
Department of Urology, Marienhospital, Hamburg, Federal Republic of Germany.
Prostate. 1989;14(3):237-49. doi: 10.1002/pros.2990140305.
A monoclonal antibody to a protein with a single band in the 30-kD region was obtained from fusion of Balb/C mouse spleen cells immunized with epithelial fractions of BPH, with the myeloma cell line P3 x 63 Ag8 6.5.3 by standard procedures. This antigen was characterized in immunobinding studies with various cellular and target antigens and in immunoperoxidase staining.
通过标准程序，将用良性前列腺增生上皮部分免疫的Balb/C小鼠脾细胞与骨髓瘤细胞系P3 x 63 Ag8 6.5.3融合，获得了一种针对在30-kD区域有单一条带的蛋白质的单克隆抗体。该抗原在与各种细胞和靶抗原的免疫结合研究以及免疫过氧化物酶染色中得到了表征。
