Sun Xiang-Ming, Zhang Yuan-Xing
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Biotechnol Lett. 2003 Jun;25(11):853-7. doi: 10.1023/a:1024084719927.
A method is described for estimating recombinant Chinese hamster ovary (rCHO) cell density in a packed-bed bioreactor by lactate production rate. The lactate production rate, which depended on both the cell numbers and cell growth rate, was modeled by segregating the cell population into two parts: one growing at a maximum specific growth rate and another non-growing. The individual cell in each part had the same lactate production rate. The established rate equation of lactate production matched the experimental data reasonably well and could be used to estimate the cell growth in the batch culture with microcarriers. Furthermore, in the perfusion culture of rCHO cells in a packed-bed bioreactor, the final cell density, 1.3 x 10(10) cells l(-1), estimated by lactate production rate, was comparable to the direct sample counting of 1.2 x 10(10) cells l(-1), showing that lactate production rate method would be useful in tracing the cell growth in packed-bed bioreactors.
描述了一种通过乳酸产生速率估算填充床生物反应器中重组中国仓鼠卵巢（rCHO）细胞密度的方法。乳酸产生速率取决于细胞数量和细胞生长速率，通过将细胞群体分为两部分进行建模：一部分以最大比生长速率生长，另一部分不生长。每部分中的单个细胞具有相同的乳酸产生速率。所建立的乳酸产生速率方程与实验数据匹配良好，可用于估算微载体分批培养中的细胞生长。此外，在填充床生物反应器中rCHO细胞的灌注培养中，通过乳酸产生速率估算的最终细胞密度为1.3×10¹⁰个细胞/升，与直接样品计数的1.2×10¹⁰个细胞/升相当，表明乳酸产生速率法可用于追踪填充床生物反应器中的细胞生长。
