Research and Development Centre, Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad 500032, India.
ATMI LifeSciences, 10851 Louisiana Ave. S, Bloomington 55438, MN, USA.
AMB Express. 2014 Apr 25;4:25. doi: 10.1186/s13568-014-0025-z. eCollection 2014.
Vaccination is believed to be the most effective method for the prevention of infectious diseases. Thus it is imperative to develop cost effective and scalable process for the production of vaccines so as to make them affordable for mass use. In this study, performance of a novel disposable iCELLis fixed bed bioreactor system was investigated for the production of some viral vaccines like Rabies, Hepatitis-A and Chikungunya vaccines in comparison to conventional systems like the commercially available packed bed system and roller bottle system. Vero and MRC-5 cell substrates were evaluated for growth parameters in all the three systems maintaining similar seeding density, multiplicity of infection (MOI) and media components. It was observed that Vero cells showed similar growth in all the three bioreactors whereas MRC-5 cells showed better growth in iCELLis Nano system and roller bottle system. Subsequently, the virus infection and antigen production studies also revealed that for Hepatitis-A and Chikungunya iCELLis Nano bioreactor system was better to the commercial packed bed bioreactor and roller bottle systems. Although for rabies antigen production commercially available packed bed bioreactor system was found to be better. This study shows that different bioreactor platforms may be employed for viral vaccine production and iCELLis Nano is one of such new convenient and a stable platform for production of human viral vaccines.
接种疫苗被认为是预防传染病最有效的方法。因此，开发具有成本效益和可扩展的疫苗生产工艺至关重要，以使它们能够负担得起大规模使用。在这项研究中，与商业上可用的填充床系统和滚瓶系统等传统系统相比，研究了新型一次性 iCELLis 固定床生物反应器系统在生产狂犬病、甲型肝炎和基孔肯雅热等病毒疫苗方面的性能。在所有三种系统中，评估了 Vero 和 MRC-5 细胞基质的生长参数，在保持相似的接种密度、感染复数 (MOI) 和培养基成分的情况下。结果表明，Vero 细胞在所有三种生物反应器中均表现出相似的生长，而 MRC-5 细胞在 iCELLis Nano 系统和滚瓶系统中生长更好。随后的病毒感染和抗原生产研究还表明，对于甲型肝炎和基孔肯雅热，iCELLis Nano 生物反应器系统优于商业填充床生物反应器和滚瓶系统。尽管狂犬病抗原生产商用填充床生物反应器系统被发现更好。本研究表明，不同的生物反应器平台可用于病毒疫苗的生产，而 iCELLis Nano 是生产人类病毒疫苗的一种新型便捷且稳定的平台。
