激光辅助对靶向动物细胞进行显微注射。
Laser-assisted microinjection into targeted animal cells.
作者信息
Mohanty Samarendra K, Sharma Mrinalini, Gupta Pradeep K
机构信息
Biomedical Applications Section Center for Advanced Technology, Indore-452013, India.
出版信息
Biotechnol Lett. 2003 Jun;25(11):895-9. doi: 10.1023/a:1024038609045.
PMID:12889802
Abstract
A pulsed (17 nanoseconds) Nd:YAG laser (1064 nm) was used to inject impermeable dyes (propidium iodide andiodide and merocyanine 540) and a plasmid (pEGFP-N1) encoding green fluorescent protein (GFP) into human breast adenocarcinoma cells (MCF-7). The cell membrane integrity and viability were fully preserved in this laser-assisted transfer.
摘要
使用脉冲（17纳秒）钕：钇铝石榴石激光（1064纳米）将不可渗透染料（碘化丙啶和部花青540）以及编码绿色荧光蛋白（GFP）的质粒（pEGFP-N1）注入人乳腺癌细胞（MCF-7）。在这种激光辅助转移过程中，细胞膜的完整性和活力得到了充分保留。
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