Hafez H M, Jodas S
State Veterinary Laboratory, Stuttgart, Germany.
Dtsch Tierarztl Wochenschr. 1992 Dec;99(12):489-90.
The conventional culture methods of hatching eggs using shell and/or egg contents for detection of salmonella organisms give mostly unsatisfactory results. The aim of the present study is to evaluate selection of other samples and techniques of culturing hatching eggs and freshly hatched chicks. This current study provides the best evidence of Salmonella enteritidis in artificially contaminated eggs (Layer type) by using enrichment broth in empty egg shell samples in comparison to culturing samples from yolk, albumen or from shell above the air cell (with the outer shell membrane). The isolation rates could be enhanced if empty egg shell was initially filled with Buffered Pepton Water as pre-enrichment broth. Examination of organs from freshly hatched chicks revealed that crop samples give mostly higher reisolation rates.
使用蛋壳和/或蛋内容物孵化鸡蛋来检测沙门氏菌的传统培养方法大多不能令人满意。本研究的目的是评估其他样本的选择以及孵化鸡蛋和刚孵出雏鸡的培养技术。与培养来自蛋黄、蛋白或气室上方蛋壳（带外壳膜）的样本相比，本研究通过在空蛋壳样本中使用增菌肉汤，为人工污染鸡蛋（蛋鸡类型）中的肠炎沙门氏菌提供了最佳证据。如果最初在空蛋壳中装入缓冲蛋白胨水作为预增菌肉汤，分离率可能会提高。对刚孵出雏鸡的器官检查表明，嗉囊样本的再次分离率大多较高。
