Zhang Fengli, Simon Anne E
Department of Cell Biology and Molecular Genetics, University of Maryland College Park, College Park, MD 20742, USA.
Virology. 2003 Jul 20;312(1):8-13. doi: 10.1016/s0042-6822(03)00345-3.
SatC, a chimeric satellite RNA associated with Turnip crinkle virus (TCV), intensifies the symptoms of TCV on all symptomatic hosts yet attenuates the symptoms of a TCV mutant that expresses low levels of a defective coat protein (CP). We now report that TCV virion levels were substantially reduced by the presence of satC or when two amino acids were inserted at the N-terminus of the CP, resulting in similarly enhanced symptoms. Since the TCV CP is a suppressor of RNA silencing, increased levels of resultant free CP could augment silencing suppression, resulting in enhanced colonization of the plant.
SatC是一种与芜菁皱缩病毒(TCV)相关的嵌合卫星RNA,它会加剧TCV在所有有症状宿主上的症状,但会减轻表达低水平缺陷衣壳蛋白(CP)的TCV突变体的症状。我们现在报告,satC的存在或在CP的N端插入两个氨基酸时,TCV病毒粒子水平会大幅降低,导致症状同样增强。由于TCV CP是RNA沉默的抑制因子,由此产生的游离CP水平增加可能会增强沉默抑制,从而导致植物定殖增强。