“枣植原体”，一种与枣树疯病相关的新型植原体分类单元。

'Candidatus phytoplasma ziziphi', a novel phytoplasma taxon associated with jujube witches'-broom disease.

作者信息

Jung Hee-Young, Sawayanagi Toshimi, Kakizawa Shigeyuki, Nishigawa Hisashi, Wei Wei, Oshima Kenro, Miyata Shin-Ichi, Ugaki Masashi, Hibi Tadaaki, Namba Shigetou

机构信息

Laboratory of Plant Pathology, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Laboratory of Bioresource Technology, University of Tokyo, 202 Frontier Bioscience Building, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.

出版信息

Int J Syst Evol Microbiol. 2003 Jul;53(Pt 4):1037-1041. doi: 10.1099/ijs.0.02393-0.

DOI:10.1099/ijs.0.02393-0

PMID:12892123

Abstract

Phylogenetic relationships of five jujube witches'-broom (JWB) phytoplasma isolates from four different districts, and other phytoplasmas, were investigated by 16S rDNA PCR amplification and sequence analysis. The 16S rDNA sequences of any pair of the five isolates of JWB phytoplasmas were > 99.5% similar. The JWB phytoplasma 16S rDNA sequences were most closely related to that of the elm yellows (EY) phytoplasma in 16S-group VIII. Phylogenetic analysis of the 16S rDNA sequences from the JWB phytoplasma isolates, together with sequences from most of the phytoplasmas archived in GenBank, produced a tree in which the JWB isolates clustered as a discrete subgroup. The uniqueness of the JWB phytoplasma appears to be correlated with a specific insect vector (Hishimonus sellatus) and the host plant (Zizyphus jujuba), or with a specific geographical distribution. The unique properties of the JWB phytoplasma sequences clearly indicate that it represents a novel taxon, 'Candidatus Phytoplasma ziziphi'.

摘要

通过16S rDNA PCR扩增和序列分析，研究了来自四个不同地区的五种枣疯病（JWB）植原体分离株与其他植原体之间的系统发育关系。五种枣疯病植原体分离株中任意两个的16S rDNA序列相似度均>99.5%。枣疯病植原体的16S rDNA序列与16S组VIII中的榆树黄化（EY）植原体的序列关系最为密切。对枣疯病植原体分离株的16S rDNA序列以及GenBank中存档的大多数植原体序列进行系统发育分析，得到一棵树，其中枣疯病分离株聚为一个独立的亚组。枣疯病植原体的独特性似乎与特定的昆虫传播介体（角蝉）和寄主植物（枣树）相关，或者与特定的地理分布相关。枣疯病植原体序列的独特性质清楚地表明它代表一个新的分类单元，即“枣植原体（暂定种）”。