Uetanabaro A P, Wahrenburg C, Hunger W, Pukall R, Spröer C, Stackebrandt E, de Canhos V P, Claus D, Fritze D
University of Campinas, 13084-510 Campinas, SP Brazil.
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2003 Jul;53(Pt 4):1051-1057. doi: 10.1099/ijs.0.02420-0.
Twenty-two agarolytic, aerobic, spore-forming strains were characterized taxonomically by DNA-DNA reassociation experiments, riboprint analyses, 16S rDNA sequencing and phenetic similarity analyses. Based on riboprint analyses, the strains formed eight ribogroups, six of which contained 2-6 strains and two encompassed single strains. Within the multi-strain ribogroups, similarities ranged from 91-99%. Phylogenetic analyses of representatives of the eight groups by 16S rDNA sequence analysis showed that the strains were affiliated to the genus Paenibacillus, but relatedness to described Paenibacillus species was only moderate (<97.8% sequence similarity). Published DNA-DNA similarity values for most of the agarolytic strains, supplemented with new data, supported the distinctiveness of the eight ribogroups. Intragroup DNA-DNA similarity values ranged from 80 to 104%, while intergroup DNA-DNA similarities were <35%. Based on genomic distinctiveness and supported by the presence of distinguishing phenotypic properties, multi-strain groups 1 and 2 are proposed as novel species, Paenibacillus agarexedens sp. nov., nom. rev. (type strain, DSM 1327T=CIP 107437T) and Paenibacillus agaridevorans sp. nov. (type strain, DSM 1355T=CIP 107436T).
通过DNA-DNA重结合实验、核糖体印记分析、16S rDNA测序和表型相似性分析，对22株溶琼脂、需氧、产芽孢菌株进行了分类学特征鉴定。基于核糖体印记分析，这些菌株形成了8个核糖组，其中6个包含2 - 6株菌株，2个包含单株菌株。在多株核糖组内，相似性范围为91% - 99%。通过16S rDNA序列分析对这8个组的代表菌株进行系统发育分析表明，这些菌株隶属于芽孢杆菌属，但与已描述的芽孢杆菌属物种的亲缘关系仅为中等程度（序列相似性<97.8%）。补充新数据后，大多数溶琼脂菌株已发表的DNA-DNA相似性值支持了这8个核糖组的独特性。组内DNA-DNA相似性值范围为80%至104%，而组间DNA-DNA相似性<35%。基于基因组独特性并辅以独特表型特征的存在，多株菌株组1和组2被提议为新物种，即嗜琼脂芽孢杆菌新种（Paenibacillus agarexedens sp. nov.，名称修订）（模式菌株，DSM 1327T = CIP 107437T）和食琼脂芽孢杆菌新种（Paenibacillus agaridevorans sp. nov.）（模式菌株，DSM 1355T = CIP 107436T）。
