将埃希氏芽孢杆菌和几丁质分解芽孢杆菌转移至类芽孢杆菌属，并对埃希氏类芽孢杆菌新组合和几丁质分解类芽孢杆菌新组合进行修订描述
Transfer of Bacillus ehimensis and Bacillus chitinolyticus to the genus Paenibacillus with emended descriptions of Paenibacillus ehimensis comb. nov. and Paenibacillus chitinolyticus comb. nov.
作者信息
Lee Jung-Sook, Pyun Yu-Ryang, Bae Kyung Sook
机构信息
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, P O Box 115, Yusong, Daejeon 305-600, Korea.
Department of Biotechnology, Yonsei University, Seoul 120-749, Korea.
出版信息
Int J Syst Evol Microbiol. 2004 May;54(Pt 3):929-933. doi: 10.1099/ijs.0.02765-0.
The taxonomic status of Bacillus ehimensis and Bacillus chitinolyticus was examined, based on their 16S rDNA sequences, DNA-DNA hybridization and other taxonomic characteristics. A phylogenetic analysis using 16S rDNA sequences revealed that the two species belong to the genus Paenibacillus. In particular, B. ehimensis KCTC 3748(T) and B. chitinolyticus KCTC 3791(T) were found to be phylogenetically closely related to Paenibacillus koreensis YC300(T) (98.3 % sequence similarity) and Paenibacillus chinjuensis WN9(T) (95.2 % sequence similarity), respectively. DNA-DNA hybridization values between B. ehimensis KCTC 3748(T) and P. koreensis YC300(T) were less than 26 %. An experiment using Paenibacillus-specific PCR primers, PAEN515F and 1377R, revealed that B. ehimensis and B. chitinolyticus had the same amplified 16S rDNA fragment as members of the genus Paenibacillus. Accordingly, it is proposed that B. ehimensis and B. chitinolyticus be transferred to the genus Paenibacillus as Paenibacillus ehimensis comb. nov. and Paenibacillus chitinolyticus comb. nov., respectively.
基于其16S rDNA序列、DNA-DNA杂交及其他分类学特征，对埃希氏芽孢杆菌（Bacillus ehimensis）和溶几丁质芽孢杆菌（Bacillus chitinolyticus）的分类地位进行了研究。利用16S rDNA序列进行的系统发育分析表明，这两个物种属于类芽孢杆菌属（Paenibacillus）。具体而言，发现埃希氏芽孢杆菌KCTC 3748(T)和溶几丁质芽孢杆菌KCTC 3791(T)在系统发育上分别与韩国类芽孢杆菌（Paenibacillus koreensis）YC300(T)（序列相似性为98.3%）和晋州类芽孢杆菌（Paenibacillus chinjuensis）WN9(T)（序列相似性为95.2%）密切相关。埃希氏芽孢杆菌KCTC 3748(T)与韩国类芽孢杆菌YC300(T)之间的DNA-DNA杂交值小于26%。使用类芽孢杆菌特异性PCR引物PAEN515F和1377R进行的实验表明，埃希氏芽孢杆菌和溶几丁质芽孢杆菌与类芽孢杆菌属成员具有相同的16S rDNA扩增片段。因此，建议将埃希氏芽孢杆菌和溶几丁质芽孢杆菌分别作为新组合种埃希氏类芽孢杆菌（Paenibacillus ehimensis comb. nov.）和溶几丁质类芽孢杆菌（Paenibacillus chitinolyticus comb. nov.）转入类芽孢杆菌属。
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