De Graef E M, Devriese L A, Vancanneyt M, Baele M, Collins M D, Lefebvre K, Swings J, Haesebrouck F
Laboratory of Veterinary Bacteriology and Mycology, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
Int J Syst Evol Microbiol. 2003 Jul;53(Pt 4):1069-1074. doi: 10.1099/ijs.0.02549-0.
Strains from anal swabs and chronic otitis externa in dogs were shown to be phylogenetically related to the Enterococcus faecium species group. They shared a number of phenotypic characteristics with these species, but they could be easily differentiated by biochemical reactions. In addition, the canine strains were unusual in their nearly complete failure to grow on sodium azide-containing enterococci-selective media and in their Voges-Proskauer reactions (usually negative). By using 16S rRNA sequencing and DNA-DNA hybridization of representative strains, as well as tDNA interspacer gene PCR and SDS-PAGE of whole-cell proteins, the group of canine strains was shown to constitute a novel enterococcal species. The name Enterococcus canis sp. nov. is proposed for this species, with LMG 12316T (= CCUG 46666T) as the type strain. Concurrently, the taxonomic situation and nomenclatural position of Enterococcus porcinus were investigated. As no phenotypic or genotypic differences were found between this species and Enterococcus villorum, the name E. porcinus is considered to be a junior synonym of E. villorum.
犬类肛门拭子和慢性外耳炎分离出的菌株在系统发育上与粪肠球菌种组相关。它们与这些菌种具有许多表型特征，但可通过生化反应轻松区分。此外，犬类菌株的不同寻常之处在于，它们几乎完全无法在含叠氮化钠的肠球菌选择性培养基上生长，且Voges-Proskauer反应通常为阴性（通常呈阴性）。通过对代表性菌株进行16S rRNA测序和DNA-DNA杂交，以及全细胞蛋白的tDNA间隔基因PCR和SDS-PAGE分析，结果表明犬类菌株组构成了一个新的肠球菌种。为此物种提议命名为犬肠球菌（Enterococcus canis sp. nov.），模式菌株为LMG 12316T（= CCUG 46666T）。同时，对猪肠球菌的分类情况和命名地位进行了研究。由于该物种与绒毛肠球菌之间未发现表型或基因型差异，因此认为猪肠球菌这一名称是绒毛肠球菌的次异名。
