Custer Thomas G, Wagner William P, Kato Shuji, Bierbaum Veronica M, Fall Ray
Department of Chemistry and Biochemistry, University of Colorado, Boulder Colorado 80309-0215, USA.
Biotechnol Prog. 2003 Jul-Aug;19(4):1355-64. doi: 10.1021/bp025730k.
Chemical-ionization mass spectrometry (CIMS) using flow reactors is an emerging method for on-line monitoring of trace concentrations of organic compounds in the gas phase. In this study, a flow-reactor CIMS instrument, employing the H(3)O(+) cation as the ionizing reagent, was used to simultaneously monitor several volatile metabolic products as they are released into the headspace during bacterial growth in a bioreactor. Production of acetaldehyde, ethanol, acetone, butanol, acetoin, diacetyl, and isoprene by Bacillus subtilis is reported. Ion signal intensities were related to solution-phase concentrations using empirical calibrations and, in the case of isoprene, were compared with simultaneous gas chromatography measurements. Identification of volatile and semivolatile metabolites is discussed. Flow-reactor CIMS techniques should be useful for bioprocess monitoring applications because of their ability to sensitively and simultaneously monitor many volatile metabolites on-line.
使用流动反应器的化学电离质谱法（CIMS）是一种用于在线监测气相中痕量有机化合物浓度的新兴方法。在本研究中，一台以H(3)O(+)阳离子作为电离试剂的流动反应器CIMS仪器，被用于在生物反应器中细菌生长期间，同时监测几种挥发性代谢产物释放到顶空中的情况。报告了枯草芽孢杆菌产生乙醛、乙醇、丙酮、丁醇、3-羟基丁酮、双乙酰和异戊二烯的情况。使用经验校准将离子信号强度与溶液相浓度相关联，并且对于异戊二烯，将其与同步气相色谱测量结果进行了比较。讨论了挥发性和半挥发性代谢产物的鉴定。流动反应器CIMS技术因其能够灵敏且同时在线监测多种挥发性代谢产物，应可用于生物过程监测应用。
