Chen Jiann-Hwa, Hsu Wen-Bin, Chiou Chien-Shun, Chen Chun-Mei
Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan, Republic of China.
FEMS Microbiol Lett. 2003 Jul 29;224(2):277-83. doi: 10.1016/S0378-1097(03)00470-1.
Conversion of serotype from 2a to Y was demonstrated with five Shigella flexneri isolates recovered from an infected patient. When introduced into the serotype Y isolate, the glucosyltransferase (gtr) II gene of the serotype 2a isolate is capable of inducing the conversion from serotype Y to 2a. In contrast, the gtrII of the serotype Y isolate lacks the capacity to change serotype, resulting from a Cys-->Tyr substitution in its predicted protein sequence. The protein product of the gtrII gene was detected. This is the first report of serotype conversion of S. flexneri in humans, and successful detection of the protein product from a gtr gene.
从一名感染患者身上分离出的5株福氏志贺菌显示出从2a血清型向Y血清型的转变。当将2a血清型分离株的葡糖基转移酶（gtr）II基因导入Y血清型分离株时，它能够诱导从Y血清型向2a血清型的转变。相反，Y血清型分离株的gtrII由于其预测的蛋白质序列中发生了半胱氨酸到酪氨酸的替换而缺乏改变血清型的能力。检测到了gtrII基因的蛋白质产物。这是人类福氏志贺菌血清型转变以及从gtr基因成功检测到蛋白质产物的首次报道。
