建立针对志贺氏菌 O 抗原修饰基因的多重 PCR 检测方法，用于志贺氏菌分子血清分型。
Development of a multiplex PCR assay targeting O-antigen modification genes for molecular serotyping of Shigella flexneri.
机构信息
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, China CDC, P.O. Box 5, Changping, Beijing, China.
出版信息
J Clin Microbiol. 2011 Nov;49(11):3766-70. doi: 10.1128/JCM.01259-11. Epub 2011 Aug 31.
Abstract
Shigella flexneri is the major Shigella species that causes diarrheal disease in developing countries. It is further subdivided into 15 serotypes based on O-antigen structure. Serotyping of S. flexneri is important for epidemiological purposes. In this study, we developed a multiplex PCR assay targeting the O-antigen synthesis gene wzx and the O-antigen modification genes gtrI, gtrIC, gtrII, oac, gtrIV, gtrV, and gtrX for molecular serotyping of S. flexneri. The multiplex PCR assay contained eight sets of specific PCRs in a single tube and can identify 14 of the 15 serotypes (the exception being serotype Xv) of S. flexneri recognized thus far. A nearly perfect concordance (97.8%) between multiplex PCR assay and slide agglutination was observed when 358 S. flexneri strains of various serotypes were analyzed, except that 8 strains were carrying additional cryptic and/or defective serotype-specific genes. The multiplex PCR assay provides a rapid and specific method for the serotype identification of S. flexneri.
摘要
福氏志贺菌是引起发展中国家腹泻病的主要志贺氏菌血清型。它进一步根据 O 抗原结构分为 15 个血清型。福氏志贺菌的血清型鉴定对于流行病学目的很重要。在本研究中，我们开发了一种针对 O 抗原合成基因 wzx 和 O 抗原修饰基因 gtrI、gtrIC、gtrII、oac、gtrIV、gtrV 和 gtrX 的多重 PCR 检测方法，用于福氏志贺菌的分子血清型鉴定。该多重 PCR 检测方法在一个管中包含 8 组特定的 PCR，可以鉴定迄今为止已识别的 14 种福氏志贺菌血清型（除外血清型 Xv）。当分析 358 株不同血清型的福氏志贺菌菌株时，多重 PCR 检测方法与玻片凝集法之间存在几乎完全一致（97.8%），但有 8 株携带额外的隐匿性和/或缺陷型血清型特异性基因。该多重 PCR 检测方法为福氏志贺菌的血清型鉴定提供了一种快速且特异的方法。
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