Ali Khaled Said, Dorgai László, Abrahám Magdolna, Hermesz Edit
Department of Biochemistry, Faculty of Science, University of Szeged, PO Box 533, H-6701, Szeged, Hungary.
Biochem Biophys Res Commun. 2003 Aug 1;307(3):503-9. doi: 10.1016/s0006-291x(03)01206-3.
Two genes expressing 70 kDa heat shock proteins were identified in Cyprinus carpio. The sequence similarities and the intron-interrupted structure of the coding regions indicate that carp Hsc70-1 and Hsc70-2 belong to the Hsp70 cognate subfamily. The expressions of the two hsc70 genes were followed by semi-quantitative RT-PCR. Both genes are expressed under unstressed conditions in a characteristic tissue-specific manner. Inducibility of the response to elevated temperature, cold shock, and Cd treatment was investigated in the liver and muscle, in whole-animal experiments. Both genes were insensitive to or only weakly induced by the stressors, with two exceptions: Cd treatment resulted in an 11-13-fold enhanced induction of hsc70-1 in the liver and cold shock enhanced induction of hsc70-2 in the muscle by 7.5-10-fold.
在鲤科鱼类中鉴定出了两个表达70 kDa热休克蛋白的基因。编码区域的序列相似性和内含子中断结构表明，鲤鱼的Hsc70-1和Hsc70-2属于Hsp70同源亚家族。通过半定量RT-PCR跟踪这两个hsc70基因的表达。这两个基因在非应激条件下均以特征性的组织特异性方式表达。在全动物实验中，研究了肝脏和肌肉对高温、冷休克和镉处理的诱导反应。这两个基因对应激源不敏感或仅受到微弱诱导，但有两个例外：镉处理导致肝脏中hsc70-1的诱导增强11-13倍，冷休克使肌肉中hsc70-2的诱导增强7.5-10倍。
