Malark J A, Peyton L C, Galvin M J
Department of Surgical Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610-0125.
J Am Vet Med Assoc. 1992 Nov 15;201(10):1545-8.
Peritoneal fluid and blood was collected from 8 healthy adult horses. Four 1-ml aliquots of peritoneal fluid from each horse were then contaminated with 0 ml (normal), 0.05 ml (1 drop), 0.10 ml (2 drops), and 0.20 ml (4 drops) of blood from the same horse. Samples were analyzed for RBC count, nucleated blood cell count, total protein concentration, and nucleated cell differential count. Statistical analysis revealed no significant changes in nucleated cell number, nucleated cell differential, or total protein concentration in peritoneal samples contaminated with blood. The RBC count significantly increased with blood contamination. It was concluded that up to 17% blood contamination of peritoneal fluid in clinically normal horses did not significantly alter interpretation of the nucleated cell count or protein concentration.
从8匹健康成年马身上采集了腹腔液和血液。然后，从每匹马身上取4份1毫升的腹腔液等分试样，分别加入0毫升（正常）、0.05毫升（1滴）、0.10毫升（2滴）和0.20毫升（4滴）来自同一匹马的血液进行污染。对样本进行红细胞计数、有核血细胞计数、总蛋白浓度和有核细胞分类计数分析。统计分析表明，被血液污染的腹腔样本中，有核细胞数量、有核细胞分类或总蛋白浓度没有显著变化。随着血液污染，红细胞计数显著增加。得出的结论是，临床正常马匹的腹腔液中血液污染率高达17%时，对有核细胞计数或蛋白质浓度的解读没有显著改变。
