Schumacher J, Spano J S, Moll H D
J Am Vet Med Assoc. 1985 Jun 15;186(12):1301-3.
Peritoneal fluid was collected from 15 clinically normal horses and was analyzed for nucleated cell (NC) counts and specific gravity. Six horses (controls, group 1) were subjected to abdominocentesis only, with a teat cannula, every 24 hours for 5 days. There were no marked changes in the peritoneal fluid of these horses over the 5-day period. Peritoneal fluid was collected from 6 other horses (group 2) with an 8.89-cm 18-gauge needle. The needle was then advanced until intestinal fluid was obtained. Peritoneal fluid was then collected with teat cannulas at 24-hour intervals for an additional 4 days. Peritoneal fluid NC counts from group 2 horses were significantly increased (P less than 0.05) at peak values 2 days after enterocentesis. Specific gravities of peritoneal fluid from group 2 horses were increased on days 1 and 2 after enterocentesis (P greater than 0.05). Peritoneal fluid from 3 other horses (group 3) was collected before enterocentesis (base line) and again at 4-hour intervals after enterocentesis. Peritoneal fluid NC counts of group 3 horses were markedly increased above base-line values 4 hours after enterocentesis and continued to increase for up to 12 hours after enterocentesis when the experiment was terminated. All horses that underwent enterocentesis remained clinically normal except 1 group 3 horse that had a fever (39.6 C) 24 hours after enterocentesis.
从15匹临床正常的马身上采集腹腔液，分析其中有核细胞（NC）计数和比重。6匹马（对照组，第1组）仅用乳头套管每24小时进行一次腹腔穿刺，持续5天。在这5天期间，这些马的腹腔液没有明显变化。从另外6匹马（第2组）用一根8.89厘米长的18号针头采集腹腔液。然后将针头推进直至获得肠液。随后用乳头套管每隔24小时再采集4天腹腔液。第2组马的腹腔液NC计数在肠穿刺后2天达到峰值时显著增加（P小于0.05）。第2组马的腹腔液比重在肠穿刺后第1天和第2天增加（P大于0.05）。从另外3匹马（第3组）在肠穿刺前（基线）以及肠穿刺后每隔4小时采集腹腔液。第3组马的腹腔液NC计数在肠穿刺后4小时显著高于基线值，并在实验终止时肠穿刺后持续增加长达12小时。除了1匹第3组马在肠穿刺后24小时发烧（39.6摄氏度）外，所有接受肠穿刺的马临床仍保持正常。
