Le Roch Karine G, Zhou Yingyao, Blair Peter L, Grainger Muni, Moch J Kathleen, Haynes J David, De La Vega Patricia, Holder Anthony A, Batalov Serge, Carucci Daniel J, Winzeler Elizabeth A
Department of Cell Biology ICND202, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Science. 2003 Sep 12;301(5639):1503-8. doi: 10.1126/science.1087025. Epub 2003 Jul 31.
The completion of the genome sequence for Plasmodium falciparum, the species responsible for most malaria human deaths, has the potential to reveal hundreds of new drug targets and proteins involved in pathogenesis. However, only approximately 35% of the genes code for proteins with an identifiable function. The absence of routine genetic tools for studying Plasmodium parasites suggests that this number is unlikely to change quickly if conventional serial methods are used to characterize encoded proteins. Here, we use a high-density oligonucleotide array to generate expression profiles of human and mosquito stages of the malaria parasite's life cycle. Genes with highly correlated levels and temporal patterns of expression were often involved in similar functions or cellular processes.
导致大多数人类疟疾死亡的恶性疟原虫的基因组序列完成后，有可能揭示数百个新的药物靶点以及与发病机制有关的蛋白质。然而，只有约35%的基因编码具有可识别功能的蛋白质。缺乏用于研究疟原虫的常规遗传工具表明，如果使用传统的系列方法来鉴定编码的蛋白质，这一数字不太可能迅速改变。在此，我们使用高密度寡核苷酸阵列来生成疟原虫生命周期中人类和蚊子阶段的表达谱。表达水平和时间模式高度相关的基因通常参与相似的功能或细胞过程。
