通过基因组、转录组和蛋白质组分析对疟原虫生命周期进行全面调查。
A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses.
作者信息
Hall Neil, Karras Marianna, Raine J Dale, Carlton Jane M, Kooij Taco W A, Berriman Matthew, Florens Laurence, Janssen Christoph S, Pain Arnab, Christophides Georges K, James Keith, Rutherford Kim, Harris Barbara, Harris David, Churcher Carol, Quail Michael A, Ormond Doug, Doggett Jon, Trueman Holly E, Mendoza Jacqui, Bidwell Shelby L, Rajandream Marie-Adele, Carucci Daniel J, Yates John R, Kafatos Fotis C, Janse Chris J, Barrell Bart, Turner C Michael R, Waters Andrew P, Sinden Robert E
机构信息
Pathogen Sequencing Unit, Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge CB10 1SA, UK.
出版信息
Science. 2005 Jan 7;307(5706):82-6. doi: 10.1126/science.1103717.
Plasmodium berghei and Plasmodium chabaudi are widely used model malaria species. Comparison of their genomes, integrated with proteomic and microarray data, with the genomes of Plasmodium falciparum and Plasmodium yoelii revealed a conserved core of 4500 Plasmodium genes in the central regions of the 14 chromosomes and highlighted genes evolving rapidly because of stage-specific selective pressures. Four strategies for gene expression are apparent during the parasites' life cycle: (i) housekeeping; (ii) host-related; (iii) strategy-specific related to invasion, asexual replication, and sexual development; and (iv) stage-specific. We observed posttranscriptional gene silencing through translational repression of messenger RNA during sexual development, and a 47-base 3' untranslated region motif is implicated in this process.
伯氏疟原虫和查巴迪疟原虫是广泛使用的疟疾模型物种。将它们的基因组与蛋白质组学和微阵列数据相结合，并与恶性疟原虫和约氏疟原虫的基因组进行比较，结果显示在14条染色体的中央区域有一个由4500个疟原虫基因组成的保守核心，并突出了由于阶段特异性选择压力而快速进化的基因。在寄生虫的生命周期中，基因表达有四种明显的策略：（i）管家基因；（ii）与宿主相关的基因；（iii）与入侵、无性繁殖和有性发育相关的特定策略基因；以及（iv）阶段特异性基因。我们观察到在有性发育过程中通过信使核糖核酸的翻译抑制实现转录后基因沉默，并且一个47个碱基的3'非翻译区基序参与了这一过程。
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