拟南芥的全基因组插入诱变
Genome-wide insertional mutagenesis of Arabidopsis thaliana.
作者信息
Alonso José M, Stepanova Anna N, Leisse Thomas J, Kim Christopher J, Chen Huaming, Shinn Paul, Stevenson Denise K, Zimmerman Justin, Barajas Pascual, Cheuk Rosa, Gadrinab Carmelita, Heller Collen, Jeske Albert, Koesema Eric, Meyers Cristina C, Parker Holly, Prednis Lance, Ansari Yasser, Choy Nathan, Deen Hashim, Geralt Michael, Hazari Nisha, Hom Emily, Karnes Meagan, Mulholland Celene, Ndubaku Ral, Schmidt Ian, Guzman Plinio, Aguilar-Henonin Laura, Schmid Markus, Weigel Detlef, Carter David E, Marchand Trudy, Risseeuw Eddy, Brogden Debra, Zeko Albana, Crosby William L, Berry Charles C, Ecker Joseph R
机构信息
Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
出版信息
Science. 2003 Aug 1;301(5633):653-7. doi: 10.1126/science.1086391.
Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the approximately 29,454 predicted Arabidopsis genes. Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels. Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.
在参考植物拟南芥的基因组中已产生超过225,000个独立的农杆菌转移DNA（T-DNA）插入事件，这些事件代表了基因空间的近乎饱和。确定了超过88,000个T-DNA插入的精确位置，这导致在大约29,454个预测的拟南芥基因中的超过21,700个基因中鉴定出突变。对整合事件分布的全基因组分析揭示了在染色体和基因水平上都存在较大的整合位点偏差。在响应植物激素乙烯而被调控的基因中鉴定出插入突变。
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