Dunger A, Wachlin G, Scharlau G
Institute of Diabetes Gerhardt Katsch, University of Greifswald, Karlsburg, Germany.
Int J Pancreatol. 1992 Dec;12(3):245-51. doi: 10.1007/BF02924364.
Recently, human amniotic fluid (HAF) from healthy women was found to stimulate growth and function of pancreatic B-cells. Here, the effect of HAF and serum from healthy probands (HS) was compared with that from probands with gestational (GD), noninsulin-dependent (NIDDM), or insulin-dependent diabetes (IDDM) on islet function and replication. Rat islets were cultured in the presence of either HAF or HS for 7 d. Insulin content and basal insulin release were not different after exposure of the islets to HAF or HS from healthy or diabetic women. In contrast to HS, HAF provoked the islets to deliver significantly more insulin during culture. Additionally, the same islets exhibited a more intense response to a glucose challenge. The degree of HAF-induced insulin release was not influenced by the type of diabetes. HAF and HS from GD and NIDDM women did not influence the islet DNA synthesis in comparison to HAF and HS from healthy pregnant women. However, HAF but not HS from IDDM pregnant women, elicited a significant increase in islet replication. Most effective in stimulating islet cell replication were HAFs from IDDM pregnant women belonging to the White D-type. It was shown that the relatively high concentration of insulin in the HAFs was not directly responsible for the observed increase of the islet DNA synthesis. HAF from women with long-term diabetes is supposed to contain factor(s) that might directly or indirectly enhance islet replication.
最近发现，健康女性的人羊水（HAF）能刺激胰腺β细胞的生长和功能。在此，将健康受试者（HS）的HAF和血清与患有妊娠期（GD）、非胰岛素依赖型（NIDDM）或胰岛素依赖型糖尿病（IDDM）的受试者的HAF和血清对胰岛功能及增殖的影响进行了比较。将大鼠胰岛在HAF或HS存在的情况下培养7天。将胰岛暴露于健康或糖尿病女性的HAF或HS后，胰岛素含量和基础胰岛素释放并无差异。与HS不同，HAF促使胰岛在培养期间释放显著更多的胰岛素。此外，相同的胰岛对葡萄糖刺激表现出更强烈的反应。HAF诱导的胰岛素释放程度不受糖尿病类型的影响。与健康孕妇的HAF和HS相比，GD和NIDDM女性的HAF和HS不影响胰岛DNA合成。然而，IDDM孕妇的HAF而非HS能引起胰岛增殖显著增加。刺激胰岛细胞增殖最有效的是属于白种D型的IDDM孕妇的HAF。结果表明，HAF中相对较高浓度的胰岛素并非观察到的胰岛DNA合成增加的直接原因。长期糖尿病女性的HAF被认为含有可能直接或间接增强胰岛增殖的因子。
