磷脂酶Cβ3的原位RNA-RNA杂交显示神经内分泌肿瘤中无表达。
In situ RNA-RNA hybridisation of phospholipase C beta 3 shows lack of expression in neuroendocrine tumours.
作者信息
Stålberg Peter, Granberg Dan, Carling Tobias, Wilander Erik, Eriksson Barbro, Gobl Anders, Akerström Goran, Rastad Jonas, Modlin Irvin M, Oberg Kjell, Skogseid Britt
机构信息
Department of Surgical Sciences, University Hospital, S-751 85 Uppsala, Sweden.
出版信息
Anticancer Res. 2003 May-Jun;23(3B):2227-32.
BACKGROUND
Phospholipase C beta 3 (PLCB3) plays an important role in the signal transduction of the seven transmembrane receptors. The gene is located in the vicinity of the Multiple Endocrine Neoplasia type 1 (MEN1) gene on chromosome 11q13. Transfection of PLCB3 to neuroendocrine cell lines lacking expression suppresses the neoplastic phenotype and affects the gene expression of S100A3 and human mismatch repair protein, suggesting a role for PLCB3 in neuroendocrine tumorigenesis.
MATERIALS AND METHODS
We used RNA-RNA in situ hybridisation for PLCB3 on a total of 82 samples including 34 from MEN1 patients.
RESULTS
We show that the PLCB3 transcript is missing in 8 out of 14 MEN1-associated neoplasias as well as in 4 out of 10 bronchial carcinoids, 2 out of 10 exocrine pancreatic cancers and one sporadic adrenocortical carcinoma.
CONCLUSION
Low or lack of PLCB3 expression in a subset of endocrine tumours, together with earlier published in vitro data on suppressor characteristics upon transfection, indicate that PLCB3 could be involved in the tumorigenesis in a subset of endocrine tumours.
背景
磷脂酶Cβ3（PLCB3）在七跨膜受体的信号转导中起重要作用。该基因位于11号染色体q13上的多发性内分泌肿瘤1型（MEN1）基因附近。将PLCB3转染到缺乏表达的神经内分泌细胞系中可抑制肿瘤表型，并影响S100A3和人类错配修复蛋白的基因表达，提示PLCB3在神经内分泌肿瘤发生中起作用。
材料与方法
我们对总共82个样本进行了PLCB3的RNA-RNA原位杂交，其中包括34个来自MEN1患者的样本。
结果
我们发现，在14个与MEN1相关的肿瘤中有8个、10个支气管类癌中有4个、10个外分泌性胰腺癌中有2个以及1个散发性肾上腺皮质癌中，PLCB3转录本缺失。
结论
一部分内分泌肿瘤中PLCB3表达低或缺乏，以及早期发表的关于转染后抑制特性的体外数据表明，PLCB3可能参与一部分内分泌肿瘤的肿瘤发生。
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