Ditteová G, Velebný S, Hrckova G
Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 040 01 Kosice, Slovak Republic.
J Helminthol. 2003 Sep;77(3):219-26. doi: 10.1079/JOH2002161.
The effects of glucan and liposomized glucan, alone or co-administered with vitamin C, and empty liposomes on hepatic fibrosis in mice infected with Mesocestoides corti (M. vogae) tetrathyridia were studied. Preparations were administered every third day from day 7 to day 31 post-infection (p.i.), nine doses in total. Activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and cholesterol levels were measured in sera collected on days 11, 15, 21, 28, 32, 42, 50 and 65 p.i. Liver fibrosis was studied on the same days by measuring hydroxyproline concentration, which is considered a marker for collagen content. Larvicidal effects of the glucan and liposome preparations were estimated on day 65 p.i. in the liver and peritoneal cavity. Glucan formulations significantly enhanced collagen content, most prominently after administration of liposomized glucan in combination with vitamin C. Activities of both enzymes and cholesterol levels were slightly modified after administration of glucan alone. Liposomized glucan with vitamin C significantly increased ALT and AST activity and cholesterol levels up to days 28-32 p.i., after which they plateaued or declined. The most pronounced decrease was after administration of liposomized glucan and vitamin C. The same pattern of biochemical parameters in serum was observed after administration of empty liposomes, however, collagen content was not modified significantly. Larval counts in the liver and the peritoneal cavity were significantly reduced after treatment with either glucan formulation, but were unaffected following treatment with empty liposomes. In summary, intense fibrosis in the liver of mice treated with liposomized glucan and vitamin C did not result in the most extensive parenchymal cell injury but, rather in the highest efficacy of treatment. Liposomal lipids were probably utilized in the reparation of the damaged parenchymal cells, while glucan stimulated phagocytic cells.
研究了葡聚糖和脂质体化葡聚糖单独或与维生素C共同给药以及空脂质体对感染柯氏中殖孔绦虫（沃氏中殖孔绦虫）四膜幼虫的小鼠肝纤维化的影响。从感染后第7天至第31天，每隔一天给药一次，共给药9次。在感染后第11、15、21、28、32、42、50和65天采集的血清中测量丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）活性和胆固醇水平。在同一天通过测量羟脯氨酸浓度来研究肝纤维化，羟脯氨酸浓度被认为是胶原蛋白含量的标志物。在感染后第65天评估葡聚糖和脂质体制剂在肝脏和腹腔中的杀幼虫效果。葡聚糖制剂显著增加了胶原蛋白含量，在脂质体化葡聚糖与维生素C联合给药后最为明显。单独给药葡聚糖后，两种酶的活性和胆固醇水平略有改变。脂质体化葡聚糖与维生素C在感染后第28 - 32天显著增加了ALT和AST活性以及胆固醇水平，之后它们趋于平稳或下降。最显著的下降是在脂质体化葡聚糖和维生素C给药后。给药空脂质体后观察到血清中生化参数的相同模式，然而，胶原蛋白含量没有显著改变。用任何一种葡聚糖制剂治疗后，肝脏和腹腔中的幼虫数量显著减少，但用空脂质体治疗后未受影响。总之，用脂质体化葡聚糖和维生素C治疗的小鼠肝脏中的强烈纤维化并未导致最广泛的实质细胞损伤，而是导致了最高的治疗效果。脂质体脂质可能用于修复受损的实质细胞，而葡聚糖刺激吞噬细胞。
