Iwagami M, Rajapakse R P V J, Paranagama W, Agatsuma T
Department of Environmental Health Science, Kochi Medical School, Oko, Nankoku City, Kochi 783-8505, Japan.
J Helminthol. 2003 Sep;77(3):239-45. doi: 10.1079/JOH2003180.
Metacercariae of Paragonimus spp. were obtained from field-collected freshwater crabs in Sri Lanka. Genomic DNA was extracted from single metacercariae. Two gene regions (partial mitochondrial cytochrome c oxidase subunit 1 (CO1) and the second internal transcribed spacer of the nuclear ribosomal gene repeat (ITS2)) were amplified using the polymerase chain reaction. Two differing sequences were obtained for each of these gene regions. Phylogenetic analyses placed the type 1 sequences as sister to a clade containing P. westermani and P. siamensis whereas the type 2 sequences were close to published sequences of P. siamensis from Thailand. The possible taxonomic status of these two types are discussed. This is the first report of molecular data about Paragonimus from Sri Lanka.
肺吸虫属的后尾蚴是从斯里兰卡野外采集的淡水蟹中获得的。从单个后尾蚴中提取基因组DNA。使用聚合酶链反应扩增两个基因区域（部分线粒体细胞色素c氧化酶亚基1（CO1）和核糖体基因重复序列的第二个内部转录间隔区（ITS2））。这两个基因区域各自获得了两个不同的序列。系统发育分析将1型序列置于包含卫氏并殖吸虫和暹罗并殖吸虫的一个分支的姐妹位置，而2型序列与来自泰国的暹罗并殖吸虫的已发表序列接近。讨论了这两种类型可能的分类地位。这是关于斯里兰卡肺吸虫分子数据的首次报告。
