Thaenkham Urusa, Waikagul Jitra
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 2008 Mar;39(2):217-21.
A part of the mitochondrial cytochrome c oxidase subunit I (COI) gene and the nuclear ribosomal DNA second internal transcribed spacer 2 (ITS2) of a newly described lung fluke, Paragonimus pseudoheterotremus, were sequenced and compared with P. heterotremus, the species with a similar morphology. Pairwise distance of COI sequences revealed a genetic difference between P. heterotremus and P pseudoheterotremus with a nucleotide difference of COI sequences between these two species of 10.6%. The constructed phylogenic tree with high bootstrap proportion suggested that P. pseudoheterotremus is a sister species of P. heterotremus.
对新描述的肺吸虫——拟异盘并殖吸虫的线粒体细胞色素c氧化酶亚基I（COI）基因的一部分以及核糖体DNA第二内部转录间隔区2（ITS2）进行了测序，并与形态相似的异盘并殖吸虫进行了比较。COI序列的成对距离揭示了异盘并殖吸虫和拟异盘并殖吸虫之间的遗传差异，这两个物种之间COI序列的核苷酸差异为10.6%。构建的具有高自展比例的系统发育树表明，拟异盘并殖吸虫是异盘并殖吸虫的姐妹物种。
