Tienthai Paisan, Kimura Naoko, Heldin Paraskevi, Sato Eimei, Rodriguez-Martinez Heriberto
Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences (SLU), SE-750 07 Uppsala, Sweden.
Reprod Fertil Dev. 2003;15(1-2):99-105. doi: 10.1071/rd02100.
Hyaluronan (HA) has been related to fertilization and embryo development in the pig. Furthermore, HA is present in pig oviduct fluid and the lining epithelium, particularly of the pre-ovulatory sperm reservoir. Because the mechanisms that regulate HA synthesis have not yet been clarified, semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was conducted to assess the expression of mRNAs of two HA-synthesizing enzymes (has2 and has3) in the oviduct epithelium (uterotubal junction, isthmus, ampullary-isthmic junction and ampulla segments) of non-inseminated (control) and inseminated (treatment) sows at pre-, peri- and post-ovulatory oestrus. Only has3 mRNA was detected; it was present in all tubal segments of both control and treatment samples. The level of has3 expression did not vary significantly between non-inseminated and inseminated specimens, but there was a tendency (NS) for increased mean values during the peri- and post-ovulatory stages compared with pre-ovulation. It is concluded that has3 is expressed by the porcine endosalpinx epithelium and the levels of expression do not vary during the critical periods of sperm transport and fertilization, despite fluctuating levels of HA in the tubal fluid at corresponding periods.
透明质酸（HA）与猪的受精和胚胎发育有关。此外，HA存在于猪输卵管液和衬里上皮中，特别是排卵前精子储存部位。由于调节HA合成的机制尚未阐明，因此进行了半定量逆转录-聚合酶链反应（RT-PCR），以评估未授精（对照）和授精（处理）母猪在排卵前、排卵期间和排卵后发情期输卵管上皮（子宫输卵管交界处、峡部、壶腹-峡部交界处和壶腹部节段）中两种HA合成酶（has2和has3）的mRNA表达。仅检测到has3 mRNA；它存在于对照和处理样本的所有输卵管节段中。未授精和授精样本之间has3的表达水平没有显著差异，但与排卵前相比，排卵期间和排卵后阶段的平均值有增加的趋势（无统计学意义）。结论是，猪输卵管内膜上皮表达has3，尽管在相应时期输卵管液中HA水平波动，但在精子运输和受精的关键时期，其表达水平没有变化。
