Hunter R H
J Reprod Fertil. 1984 Sep;72(1):203-11. doi: 10.1530/jrf.0.0720203.
Using the surgical approach of post-coital ligation and transection of the distal oviduct at different times relative to ovulation, together with subsequent recovery of the eggs, gilts mated at the onset of oestrus were studied for progression of viable spermatozoa within the isthmus. Results are derived from 76 animals and examination of 1047 eggs. Transection of the isthmus 1.5-2.0 cm proximal to the utero-tubal junction at intervals from 3 to 36 h after mating prevented fertilization in 269 of 270 eggs, whereas 98% of 223 eggs were fertilized in the control oviducts. Transection at 38 h (pre-ovulatory), 40 h (peri-ovulatory) and 42-44 h (post-ovulatory) after mating yielded, respectively, 5%, 40% and 100% fertilization. The mean number of spermatozoa associated with the zona pellucida increased in a parallel manner. These results, and those obtained with ligatures placed closer to the site of fertilization just after ovulation, indicate a pre-ovulatory arrest of viable spermatozoa in the caudal region of the isthmus for 36 h or more followed by an active ad-ovarian redistribution.
采用性交后结扎并在相对于排卵的不同时间横断输卵管远端的手术方法，以及随后回收卵子，对在发情开始时交配的后备母猪进行了研究，以观察峡部内活精子的进展情况。结果来自76只动物，并检查了1047枚卵子。在交配后3至36小时，在子宫输卵管连接处近端1.5 - 2.0厘米处横断峡部，270枚卵子中有269枚未受精，而在对照输卵管中，223枚卵子中有98%受精。在交配后38小时（排卵前）、40小时（排卵时）和42 - 44小时（排卵后）横断峡部，受精率分别为5%、40%和100%。与透明带相关的精子平均数量以平行方式增加。这些结果，以及在排卵后立即在更靠近受精部位处结扎所获得的结果，表明活精子在峡部尾部区域排卵前停滞36小时或更长时间，随后进行活跃的向卵巢侧重新分布。
