Yanamoto Hiroji, Nagata Izumi, Niitsu Yoichi, Xue Jing Hui, Zhang Zhiwen, Kikuchi Haruhiko
Laboratory for Cerebrovascular Disorders, Research Institute of the National Cardio-Vascular Center, 565-8565, Suita, Japan.
Exp Neurol. 2003 Aug;182(2):261-74. doi: 10.1016/s0014-4886(03)00116-x.
The temporary three-vessel occlusion (3VO) technique with a surgical approach for middle cerebral artery (MCA) produces consistent cerebral infarction in the neocortex in normotensive rats. The intraluminal thread-occlusion technique with an endovascular approach targeting the MCA occlusion (MCAO) is more widely used since it does not require complicated intracranial procedures. The aim of this study was to review the methods/models for MCAO stroke in normotensive rats and to evaluate a 3VO stroke model that provides consistent degrees and variance of cortical stroke injury for additional discussion. First, we analyzed a model with modified temporary 3VO technique requiring less complicated procedures than the temporary 3VO model, i.e., temporary occlusion of the bilateral common carotid arteries (CCAs) superimposed on a permanent occlusion of the MCA, in Sprague-Dawley rats or C57BL/6J mice. In the microvascular tissue (cerebral) perfusion study, significant reductions in regional cerebral perfusion during the 3VO accompanied a rapid return to baseline after release of the CCAs, showing that the technique induces temporary focal ischemia. The average sizes and variances of the neocortical infarction in this model, together with those in the other normotensive rat models caused by the 3VO technique in the literature, indicated a standard size and variance of infarcted lesion in the control groups relative to the specific ischemic period. However, stroke injuries in the neocortex induced by the thread occlusion technique showed greater variability with less consistent lesion sizes. Inclusion/exclusion criteria to avoid inappropriate cases with too mild (no/faint infarction) or too great (huge/fatal infarction) severity in the ischemic injury may differ between laboratories in the thread occlusion model.
采用手术方法对大脑中动脉（MCA）进行临时三血管闭塞（3VO）技术，可在正常血压大鼠的新皮层产生一致的脑梗死。由于不需要复杂的颅内手术，采用血管内方法靶向MCA闭塞（MCAO）的腔内丝线闭塞技术应用更为广泛。本研究的目的是回顾正常血压大鼠MCAO中风的方法/模型，并评估一种能提供一致程度和变异性的皮质中风损伤的3VO中风模型，以供进一步讨论。首先，我们分析了一种改良的临时3VO技术模型，该模型所需程序比临时3VO模型更简单，即在Sprague-Dawley大鼠或C57BL/6J小鼠中，双侧颈总动脉（CCA）的临时闭塞叠加在MCA的永久性闭塞上。在微血管组织（脑）灌注研究中，3VO期间局部脑灌注显著降低，CCA松开后迅速恢复到基线水平，表明该技术可诱导短暂性局灶性缺血。该模型中新皮层梗死的平均大小和变异性，以及文献中其他由3VO技术导致的正常血压大鼠模型的相关数据，表明相对于特定缺血期，对照组梗死灶的大小和变异性具有标准值。然而，丝线闭塞技术诱导的新皮层中风损伤变异性更大，损伤大小一致性较差。在丝线闭塞模型中，不同实验室避免缺血损伤过轻（无/轻微梗死）或过重（巨大/致命梗死）的不当病例的纳入/排除标准可能有所不同。
