α-寡脱氧核苷酸硫代磷酸酯的合成与性质
Synthesis and property of alpha-oligodeoxynucleotide phosphorothioate.
作者信息
Shinozuka K, Hirota Y, Morita T, Sawai H
机构信息
Department of Chemistry, Faculty of Engineering, Gunma University, Kiryu City, Japan.
出版信息
Nucleic Acids Symp Ser. 1992(27):119-20.
PMID:1289786
Abstract
Unnatural DNA analog, alpha-2'-deoxy-oligoadenylate phosphorothioate bearing 3'-normal monophosphate moiety was conveniently synthesized through phosphoramidite solid support method. The starting material of the DNA analog, alpha-2'-deoxyadenosine, was prepared in a stereospecific manner by the coupling reaction between activated nucleobase and 1-chloro-2-deoxyribose derivative.
摘要
非天然DNA类似物，即带有3'-正常单磷酸基团的α-2'-脱氧寡腺苷酸硫代磷酸酯，通过亚磷酰胺固相支持法方便地合成。DNA类似物的起始原料α-2'-脱氧腺苷是通过活化的核碱基与1-氯-2-脱氧核糖衍生物之间的偶联反应以立体特异性方式制备的。
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