Wu Xiaolei, Larson Steven R, Hu Zanmin, Palazzo Antonio J, Jones Thomas A, Wang Richard R-C, Jensen Kevin B, Chatterton N Jerry
United States Department of Agriculture, Agruiculture Research Service, Fage and Range Research Laboratory, Utah State University, UT 84322-6300, USA.
Genome. 2003 Aug;46(4):627-46. doi: 10.1139/g03-048.
Molecular genetic maps were constructed for two full-sib populations, TTC1 and TTC2, derived from two Leymus triticoides x Leymus cinereus hybrids and one common Leymus triticoides tester. Informative DNA markers were detected using 21 EcoRI-MseI and 17 PstI-MseI AFLP primer combinations, 36 anchored SSR or STS primer pairs, and 9 anchored RFLP probes. The 164-sib TTC1 map includes 1069 AFLP markers and 38 anchor loci in 14 linkage groups spanning 2001 cM. The 170-sib TTC2 map contains 1002 AFLP markers and 36 anchor loci in 14 linkage groups spanning 2066 cM. Some 488 homologous AFLP loci and 24 anchor markers detected in both populations showed similar map order. Thus, 1583 AFLP markers and 50 anchor loci were mapped into 14 linkage groups, which evidently correspond to the 14 chromosomes of allotetraploid Leymus (2n = 4x = 28). Synteny of two or more anchor markers from each of the seven homoeologous wheat and barley chromosomes was detected for 12 of the 14 Leymus linkage groups. Moreover, two distinct sets of genome-specific STS markers were identified in these allotetraploid Leymus species. These Leymus genetic maps and populations will provide a useful system to evaluate the inheritance of functionally important traits of two divergent perennial grass species.
构建了两个全同胞群体TTC1和TTC2的分子遗传图谱，这两个群体源自两个小麦状赖草×灰色赖草杂交种以及一个普通的小麦状赖草测试品种。使用21对EcoRI-MseI和17对PstI-MseI AFLP引物组合、36对锚定SSR或STS引物对以及9个锚定RFLP探针检测信息性DNA标记。164个同胞的TTC1图谱包括1069个AFLP标记和14个连锁群中的38个锚定位点，跨度为2001厘摩。170个同胞的TTC2图谱包含1002个AFLP标记和14个连锁群中的36个锚定位点，跨度为2066厘摩。在两个群体中检测到的约488个同源AFLP位点和24个锚定标记显示出相似的图谱顺序。因此，1583个AFLP标记和50个锚定位点被定位到14个连锁群中，这显然对应于异源四倍体赖草的14条染色体（2n = 4x = 28）。在14个赖草连锁群中的12个中检测到了来自七个同源小麦和大麦染色体中每一个的两个或更多锚定标记的同线性。此外，在这些异源四倍体赖草物种中鉴定出了两组不同的基因组特异性STS标记。这些赖草遗传图谱和群体将提供一个有用的系统来评估两个不同多年生草种功能重要性状的遗传。
