Sugimoto N, Honda K, Sasaki M
Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Nucleic Acids Symp Ser. 1992(27):129-30.
Double-helix formation of self-complementary deoxyribooctanucleotides, d(TCTATAGA) and d(TAGATCTA), with identical nearest neighbor base pairs has been studied by means of UV melting and temperature-jump techniques. The self-complementary duplexes of both octanucleotides with identical nearest neighbors had similar stabilities: The stabilization energies of the octanucleotides at 25 degrees C were 5.8 kcal mol-1 for d(TCTATAGA) and 6.7 kcal mol-1 for d(TAGATCTA). On the kinetic curve the melting reactions finished within 20 ms for d(TCTATAGA) and 40 ms for d(TAGATCTA) at 20 degrees C. For both octanucleotides the rate constants of dissociation increased and the rate constants of association decreased with increasing temperature.
通过紫外熔解和温度跃升技术研究了具有相同相邻碱基对的自互补脱氧核糖八核苷酸d(TCTATAGA)和d(TAGATCTA)的双螺旋形成。两种具有相同相邻碱基的八核苷酸的自互补双链体具有相似的稳定性：在25℃时，八核苷酸d(TCTATAGA)的稳定能为5.8 kcal mol-1，d(TAGATCTA)的稳定能为6.7 kcal mol-1。在动力学曲线上，20℃时d(TCTATAGA)的熔解反应在20毫秒内完成，d(TAGATCTA)的熔解反应在40毫秒内完成。对于两种八核苷酸，随着温度升高，解离速率常数增加，缔合速率常数降低。
