Sugimoto N, Matsumura A, Katoh M, Sasaki M
Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Nucleic Acids Symp Ser. 1992(27):139-40.
Double-helix formations of self-complementary chimeric hexanucleotides, r(CGCGCG), r(CGCG)d(CG), r(CG)d(CGCG), and d(CGCGCG), have been studied spectrophotometrically an thermodynamically in 1 mol dm-3 NaCl buffer. CD (circular dichroism) spectra showed that r(CGCGCG), r(CGCG)d(CG), and r(CG)d(CGCG) formed A-type double helix, while d(CGCGCG) formed B-type double helix. The stabilization energies of these helices at 37 degrees C obtained from UV melting analyses were 9.2 kcal mol-1 for r(CGCGCG), 8.2 kcal mol-1 for r(CGCG)d(CG), 6.8 kcal mol-1 for r(CG)d(CGCG), and 8.5 kcal mol-1 for d(CGCGCG), respectively.
在1 mol dm-3 NaCl缓冲液中，通过分光光度法和热力学方法研究了自我互补嵌合六核苷酸r（CGCGCG）、r（CGCG）d（CG）、r（CG）d（CGCG）和d（CGCGCG）的双螺旋结构。圆二色光谱（CD）表明，r（CGCGCG）、r（CGCG）d（CG）和r（CG）d（CGCG）形成A型双螺旋，而d（CGCGCG）形成B型双螺旋。通过紫外熔解分析获得的这些螺旋在37℃时的稳定能分别为：r（CGCGCG）为9.2 kcal mol-1，r（CGCG）d（CG）为8.2 kcal mol-1，r（CG）d（CGCG）为6.8 kcal mol-1，d（CGCGCG）为8.5 kcal mol-1。
