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pUC19的β-内酰胺酶启动子之前区域的DNA构象，这是高效转录所必需的。

DNA conformation of the region preceding the beta-lactamase promoter of pUC19 which is required for efficient transcription.

作者信息

Hirota Y, Ohyama T

机构信息

Section of Molecular Biology, Meiji Institute of Health Science, Odawara, Japan.

出版信息

Nucleic Acids Symp Ser. 1992(27):151-2.

PMID:1289798
Abstract

The upstream region of the beta-lactamase promoter of Escherichia coli plasmid pUC19 has a DNA curvature (bent DNA). This region was replaced with another sequence by using randomly synthesized oligonucleotides. Among the reconstructed plasmids, a plasmid which could endow E. coli cells with the strongest resistance to ampicillin on plates was selected. Nucleotide sequence and DNA conformation of the altered region was investigated.

摘要

大肠杆菌质粒pUC19的β-内酰胺酶启动子上游区域存在DNA弯曲（弯曲DNA）。通过使用随机合成的寡核苷酸，将该区域替换为另一个序列。在重建的质粒中，选择了一个能使大肠杆菌细胞在平板上对氨苄青霉素具有最强抗性的质粒。对改变区域的核苷酸序列和DNA构象进行了研究。

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