Ohyama T, Hirota Y
Meiji Institute of Health Science, Odawara, Japan.
Nucleic Acids Symp Ser. 1993(29):153-4.
The region preceding the beta-lactamase promoter of Escherichia coli plasmid pUC19 has a DNA curvature (curved DNA or bent DNA). Studies using mutant plasmids showed that the curved DNA structure is required for efficient transcription of the beta-lactamase gene and suggested that the requisite shape is a small part of a right-handed coil. Based on these results, the possible functional significance of DNA curvature in transcription will be discussed.
大肠杆菌质粒pUC19的β-内酰胺酶启动子之前的区域存在DNA弯曲(弯曲DNA或扭曲DNA)。使用突变体质粒进行的研究表明,弯曲的DNA结构是β-内酰胺酶基因高效转录所必需的,并表明所需形状是右手螺旋的一小部分。基于这些结果,将讨论DNA弯曲在转录中可能的功能意义。