Sp1锌指对GC盒DNA的识别及结构扰动
Recognition and structural perturbation of GC box DNA by Sp1 zinc finger.
作者信息
Kuwahara J, Futamura M, Yonezawa A, Sugiura Y
机构信息
Institute for Chemical Research, Kyoto University, Japan.
出版信息
Nucleic Acids Symp Ser. 1992(27):175-6.
PMID:1289808
Abstract
Interaction of Sp1 with GC box DNA was investigated by several footprinting experiments. Methylation of four guanine bases is strongly protected by Sp1 binding, while one guanine base in GC box is extremely hypermethylated. Sp1 binding also induces new cleavage at 5'-GA-3' site within GC box by bleomycin-iron complex.
摘要
通过多个足迹实验研究了Sp1与GC盒DNA的相互作用。Sp1结合能强烈保护四个鸟嘌呤碱基的甲基化，而GC盒中的一个鸟嘌呤碱基则极度超甲基化。Sp1结合还会通过博来霉素 - 铁复合物在GC盒内的5'-GA-3'位点诱导新的切割。
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