Suyama A, Tsuchiya A, Fukuda M, Yano K
Institute of Physics, College of Arts and Sciences, University of Tokyo, Japan.
Nucleic Acids Symp Ser. 1992(27):23-4.
Temperature-gradient DNA probe chromatography of nucleic acids on a nonporous support with a homogeneous particle size of 2.5 microns showed a higher base sequence discriminating power and a larger linear capacity than that on a porous support with a larger and less homogeneous particle size. The resolution on the nonporous support was high enough to separate samples with a single-base mismatch of the less destabilizing groups, including G-G and G-T base mismatches, even when it locates very near the end of the probe.
在粒径均一为2.5微米的无孔载体上进行核酸的温度梯度DNA探针色谱分析，相较于在粒径较大且不均一的多孔载体上，显示出更高的碱基序列辨别能力和更大的线性容量。即使单碱基错配位于探针非常靠近末端的位置，无孔载体上的分辨率也足以分离具有稳定性稍低基团的单碱基错配样品，包括G-G和G-T碱基错配。
