Tsurui H, Suyama A, Wada A
Department of Physics, Faculty of Science, University of Tokyo, Japan.
Nucleic Acids Symp Ser. 1988(19):49-52.
We have developed the temperature-gradient DNA-probe column chromatography as the new method for detecting and purifying particular DNAs or RNAs accurately. The method has high discrimination resolution, and can detect a single-base change in sample base sequences. The method also has high performances in purification of particular DNAs or RNAs, which has been demonstrated by purification of bovine mitochondria serine t-RNA. None of other method can succeed in complete purification of this t-RNA molecule. The present method can be applied not only to molecular biology as basic tools for purifying and picking-up particular sequences but also to the medical field as diagnostic tools.
我们已经开发出温度梯度DNA探针柱色谱法，作为一种准确检测和纯化特定DNA或RNA的新方法。该方法具有高分辨能力，能够检测样品碱基序列中的单碱基变化。该方法在特定DNA或RNA的纯化方面也具有高性能，这已通过牛线粒体丝氨酸t-RNA的纯化得到证明。其他方法均无法成功完全纯化这种t-RNA分子。本方法不仅可以作为纯化和提取特定序列的基础工具应用于分子生物学领域，还可以作为诊断工具应用于医学领域。
