使用聚（9-乙烯基腺嘌呤）-聚丙烯酰胺共轭凝胶通过毛细管亲和凝胶电泳（CAGE）识别（d(TTTATT) 和 d(TTATTT)） 。
Recognition of (d(TTTATT) and d(TTATTT) by capillary affinity gel electrophoresis (CAGE) using poly(9-vinyladenine)-polyacrylamide conjugated gel.
作者信息
Sawa T, Akashi M, Baba Y, Tsuhako M
机构信息
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Japan.
出版信息
Nucleic Acids Symp Ser. 1992(27):51-2.
PMID:1289824
Abstract
Sequence-specific recognition of oligodeoxynucleotide isomers (TTTATT and TTATTT) was achieved by using polyacrylamide-poly(9-vinyladenine) conjugated gel filled capillary affinity gel electrophoresis. It was found that the interaction between poly(9-vinyladenine) and the isomers was dependent on the sequential thymidylic acids of them.
摘要
通过使用聚丙烯酰胺 - 聚（9 - 乙烯基腺嘌呤）共轭凝胶填充毛细管亲和凝胶电泳实现了对寡聚脱氧核苷酸异构体（TTTATT和TTATTT）的序列特异性识别。发现聚（9 - 乙烯基腺嘌呤）与这些异构体之间的相互作用取决于它们的胸腺嘧啶酸序列。
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