Baba Y, Sawa T, Kishida A, Akashi M
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, Japan.
Electrophoresis. 1998 Mar;19(3):433-6. doi: 10.1002/elps.1150190312.
Capillary affinity gel electrophoresis was applied to sequence-specific and base composition-specific recognition of oligodeoxynucleotides, utilizing the formation of heteroduplexes between a nucleic acid analogue immobilized into the capillary gel and soluble oligodeoxynucleotides with different sequences. Capillary affinity gel electrophoresis using capillaries filled with a conjugated gel of polyacrylamide and a synthetic nucleic acid analogue [poly(9-vinyladenine)] was effective for the selective separation of hexathymidylic acid from a mixture of four homopolymers of A6, C6, G6, and T6 and for the complete resolution of five heteropolymers of hexadeoxynucleotides (TAAAAA, TTAAAA, TTTAAA, TTTTAA, TTTTTA). We also demonstrated that capillary affinity gel electrophoresis was useful for the selective and the sensitive sequence-specific recognition of sequence isomers of DNA (TTTTAA, TTTTAAT, TTTATA, and TTTTAA).
毛细管亲和凝胶电泳被应用于寡脱氧核苷酸的序列特异性和碱基组成特异性识别，利用固定在毛细管凝胶中的核酸类似物与具有不同序列的可溶性寡脱氧核苷酸之间形成异源双链体。使用填充有聚丙烯酰胺和合成核酸类似物[聚（9-乙烯基腺嘌呤）]共轭凝胶的毛细管进行毛细管亲和凝胶电泳，对于从A6、C6、G6和T6四种同聚物的混合物中选择性分离六聚胸苷酸以及完全分离五种十六聚脱氧核苷酸（TAAAAA、TTAAAA、TTTAAA、TTTTAA、TTTTTA）的杂聚物是有效的。我们还证明了毛细管亲和凝胶电泳对于DNA序列异构体（TTTTAA、TTTTAAT、TTTATA和TTTTAA）的选择性和灵敏的序列特异性识别是有用的。
