体外培养的小鼠肾小球系膜细胞中葡萄糖转运蛋白1的鉴定及其功能测定
Identification of glucose transporter-1 and it's functional assay in mouse glomerular mesangial cells cultured in vitro.
作者信息
Zhang J, Liu Z, Liu D, Li L
机构信息
Research Institute of Nephrology, Jingling Hospital, Nanjing University School of Medicine, Nanjing 210002.
出版信息
Chin Med Sci J. 2001 Mar;16(1):35-9.
OBJECTIVE
To evaluate the role of glucose transporter-1 (GLUT1) in the glucose uptake of glomerular mesangial cells.
METHODS
Cultured C57/SJL mouse mesangial cells were used in the study. The expression of GLUT1 mRNA was detected by RT-PCR. The expression of GLUT1 protein was detected by immunofluorescence and flow cytometry. The uptake of glucose and its kinetics were determined by 2-deoxy-[3H] -D-glucose uptake.
RESULTS
Both GLUT1 mRNA and protein were found in mouse glomerular mesangial cells. 2-deoxy-D-glucose uptake and kinetics assay showed that this glucose transporter had high affinity for glucose and the glucose uptake specificity was further confirmed by phloretin.
CONCLUSION
Functional GLUT1 did present in mouse mesangial cells cultured in vitro and it might be the predominant transporter mediated the uptake of glucose into mesangial cells.
目的
评估葡萄糖转运蛋白1（GLUT1）在肾小球系膜细胞摄取葡萄糖过程中的作用。
方法
本研究采用培养的C57/SJL小鼠系膜细胞。通过逆转录聚合酶链反应（RT-PCR）检测GLUT1 mRNA的表达。通过免疫荧光和流式细胞术检测GLUT1蛋白的表达。采用2-脱氧-[3H]-D-葡萄糖摄取法测定葡萄糖摄取及其动力学。
结果
在小鼠肾小球系膜细胞中发现了GLUT1 mRNA和蛋白。2-脱氧-D-葡萄糖摄取及动力学分析表明，这种葡萄糖转运蛋白对葡萄糖具有高亲和力，而根皮素进一步证实了葡萄糖摄取的特异性。
结论
功能性GLUT1确实存在于体外培养的小鼠系膜细胞中，它可能是介导葡萄糖进入系膜细胞摄取的主要转运蛋白。
Zotero 插件