Chen D, Wang W, Wang J
Department of Gastrointestinal Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032.
Chin Med Sci J. 2001 Mar;16(1):46-8.
To evaluate the effects of epidermal growth factor (EGF) on intestinal permeability and bacterial translocation in rats with acute pancreatitis during total parenteral nutrition (TPN).
Thirty-two male Sprague-Dawley rats that underwent injection of 3.5% sodium taurocholate solution into the pancreatic duct were randomly divided into one of the following two groups: (1) received only TPN (control group) or (2) received TPN with EGF at a dose of 0.2 mg x kg(-1) x day(-1) (Egf group). On fifth day of total parenteral nutrition, samples from mesenteric lymph nodes, pancreas, liver and spleen were harvested for cultures. Water, protein and DNA content in jejunal mucosa were determined. D-xylose and fluorescein isothiocyanate (FTC)-dextran were instilled into the lumen of a ligated segament of small intestine. Thirty minutes later, superior mesenteric vein D-xylose and plasma FITC-dextran concentration were measured.
Positive cultures in liver and spleen, as well as FITC-dextran concentration in the Egf group were significantly lower than in the control group. Protein and DNA content in jejunal mucosa in the Egf group were significantly higher than in the control group.
The results indicate that EGF may prevent increased intestinal permeability and bacterial translocation in rats with acute pancreatitis during TPN.
评估表皮生长因子（EGF）对全胃肠外营养（TPN）期间急性胰腺炎大鼠肠通透性和细菌移位的影响。
将32只经胰管注射3.5%牛磺胆酸钠溶液的雄性Sprague-Dawley大鼠随机分为以下两组之一：（1）仅接受TPN（对照组）或（2）接受剂量为0.2mg·kg⁻¹·d⁻¹的EGF的TPN（EGF组）。在全胃肠外营养的第5天，采集肠系膜淋巴结、胰腺、肝脏和脾脏样本进行培养。测定空肠黏膜中的水、蛋白质和DNA含量。将D-木糖和异硫氰酸荧光素（FTC）-葡聚糖注入结扎的小肠段腔内。30分钟后，测量肠系膜上静脉D-木糖和血浆FITC-葡聚糖浓度。
EGF组肝脏和脾脏的阳性培养结果以及FITC-葡聚糖浓度均显著低于对照组。EGF组空肠黏膜中的蛋白质和DNA含量显著高于对照组。
结果表明，EGF可能预防TPN期间急性胰腺炎大鼠肠通透性增加和细菌移位。
