Cherid A, Cherid N, Chamlian V, Hardwigsen J, Nouhou H, Dodero F, Benkoel L, Le Treut Y P, Chamlian A
Cellular and Metabolic Pathology of Liver, INSERM U 559, Faculty of Medicine, 27 Bd Jean Moulin, 13385 Marseille Cedex, France.
Cell Mol Biol (Noisy-le-grand). 2003 Jun;49(4):509-14.
To find a prognosis model of human liver transplant, we evaluate 62 surgical biopsies for the loss of glycogen and its variations in relation to cold ischemia, reperfusion, lobular zonation and donor's ages. We applied univariate, multivariate and discriminant analysis and logistic regression. There was a clear lobular zonation of glycogen during cold ischemia and at reperfusion. During cold ischemia, the mean loss was 48% in periportal zones and 74% in pericentrilobular zones. At reperfusion, it was in the range of 60% in periportal zones and 95% in pericentrilobular zones. It was observed in 64% of the grafts for an ischemia time less than 10 hr and in 82% of the grafts for an ischemia time of 10 hr or more. It was increased by 90% at reperfusion with pericentral predominance. Donors' age was an aggravating factor of glycogen loss beyond 28 years of age. In conclusion, in periportal zones, mean global glycogen depletion was about 54% during cold ischemia and reperfusion. It decreased by 90% at reperfusion with pericentral predominance. Logistic regression has allowed modelization of cold ischemia and reperfusion.
为了找到人类肝移植的预后模型，我们评估了62份手术活检样本中糖原的损失情况及其与冷缺血、再灌注、小叶分区和供体年龄的关系。我们应用了单变量、多变量和判别分析以及逻辑回归。在冷缺血和再灌注期间，糖原存在明显的小叶分区现象。在冷缺血期间，门周区的平均损失率为48%，中央周围区为74%。在再灌注时，门周区的损失率在60%左右，中央周围区为95%。缺血时间小于10小时的移植物中，64%出现这种情况；缺血时间为10小时或更长时间的移植物中，82%出现这种情况。再灌注时，以中央周围为主的情况下，糖原损失增加了90%。供体年龄超过28岁是糖原损失的一个加重因素。总之，在门周区，冷缺血和再灌注期间的平均总体糖原消耗约为54%。以中央周围为主的再灌注时，糖原消耗减少了90%。逻辑回归使得能够对冷缺血和再灌注进行建模。
