Dodero F, Benkoel L, Allasia C, Hardwigsen J, Campan P, Botta-Fridlund D, LE Treut Y P, Chamlian A
Laboratory of Cellular and Metabolic Pathology of Liver and INSERM U 260, Faculty of Medicine, Marseille, France.
Cell Mol Biol (Noisy-le-grand). 2000 Nov;46(7):1157-61.
In order to examine glucose metabolism in liver grafts after cold ischemia and reperfusion, the heterogeneous lobular distribution pattern of glycogen content was studied using histochemical quantitative analysis. In most of the cases, this heterogeneous pattern of glycogen was observed after preservation and reperfusion. However, a 42% reduction of glycogen content, expressed as the ratio between stained surface and total surface of liver biopsies, was observed in biopsies after reperfusion. Moreover, both periportal and centrilobular hepatocytes showed a significant decrease in mean optical density after reperfusion (18% and 25%, respectively). The comparison of our results to early postoperative liver function tests and cold ischemia times showed no significant correlation (p<0.05).
为了研究冷缺血和再灌注后肝移植中葡萄糖代谢情况，采用组织化学定量分析方法研究了糖原含量的异质性小叶分布模式。在大多数情况下，保存和再灌注后观察到糖原的这种异质性模式。然而，再灌注后的肝活检中，以肝活检染色面积与总面积之比表示的糖原含量降低了42%。此外，再灌注后门周和小叶中心肝细胞的平均光密度均显著降低（分别为18%和25%）。将我们的结果与术后早期肝功能检查及冷缺血时间进行比较，未发现显著相关性（p<0.05）。
